Demon King Piccolo spawned Tambourine shortly after his release at the hands of Emperor Pilaf. He was sent out in order to kill any of the martial artists that entered the previous two World Martial Arts Tournaments and therefore get rid of anyone who could learn how to use the Evil Containment Wave, the technique that sealed Piccolo in a rice cooker during his previous reign of terror, which the Namekian believed was the only thing that could defeat him. Tambourine was also instructed to bring back any Dragon Balls that he found along the way.
He first killed Kuririn, whom he encountered at the end of the 22nd Tenkaichi Budokai and easily managed to kill the boy with a kick to the head that snapped his neck. The mutant Namekian then stole the Four Star Dragon Ball, along with a list of all the contestants.
Tambourine was later confronted by Son Goku, who sought to avenge Kuririn. He easily managed to defeat Goku, because the boy was weakened from his fight with Tenshinhan.
Tambourine proceeded to beat the boy senseless, leaving for him dead, and destroyed his source of transport, the Kinto'un cloud. The mutant Namekian went on to kill Giran, Bacterian,
Panpoot, King Chapa, Man-Wolf, and many others. Before Tambourine could eliminate Yamcha, he received a telepathic message from Piccolo to find and destroy the person responsible for killing another of Piccolo's spawn Cymbal. Tambourine was successful in tracking down Yajirobe, who had killed Cymbal, but when Goku appeared, the mutant became more interested in fighting Goku instead. Tambourine started the match, confident he could win again. This time however, Goku managed to pound Tambourine into the ground and when he attempted to escape, destroyed him with a Kamehameha wave.
◾Like Demon King Piccolo and all of his offspring, Tambourine is named after a percussion instrument, the tambourine.
◾During Demon King Piccolo's first reign of terror, he produced another son that bore a great
Two of Piccolo's unnamed offspring, which greatly resemble Tambourine and Cymbal ◾Tambourine has the highest body count out of all the henchmen in Dragon Ball, with a total of 50 kills, maybe more.